RNA干涉中的特殊酶及实现RNA干涉的策略

The special enzyme and realization strategy of RNA intervene

RNA干涉技术已广泛地应用到功能基因组学,药物靶点筛选,细胞信号传导通路分析,疾病治疗等多方面,在多数真核细胞中RNA干涉对基因表达的抑制作用是通过21～23nt大小的siRNA实现的,后者以碱基互补的作用,识别并最终诱导mRNA降解,在此过程中有多种酶参与并协同调节。防止酶污染获得高纯度的dsRNA或siRNA是实现RNA干涉最重要的一步。介绍了设计dsRNA或siRNA几种常用并已成熟的方法,目的是帮助人们更好地应用RNA干涉技术揭示生命的奥秘。

RNA intervene had been widely applied to functional genomics,drug targtets filter,signal transduction and disease therapy.RNA intervene restrainted gene expression in most eucaryotic cells by siRNA with 21～23nt.The 1atter identified and eventually induced mRNA's degradation through the effect of base pairing,and in this coures many enzymes took part in and cooprated with each other.To prevent enzyme pollution and obtain highly pure dsRNA or siRNA was the most important step of realizing RNA intervene.Several methods of the design of dsRNA or siRNA which were generally used,the purpose was to help people apply RNA intervene technology to discover the arcanum of lives better.