植物化的猪α-乳清蛋白基因人工合成

Synthesis of Plantified Porcine Alpha-Lactalbumin Gene

根据猪α-乳清蛋白基因 c DNA序列设计了多对特异性引物 ,采用循环 PCR方法人工合成了植物化的猪α-乳清蛋白基因编码区 (398bp)并对该 PCR产物进行测序。DNA序列测定结果表明 ,采用循环 PCR法扩增的产物是预期的猪 α-乳清蛋白基因编码区 ,该植物化的猪 α-乳清蛋白基因编码区可以用于植物转化 ,为猪 α-乳清蛋白基因转基因植物研究和在转基因植物中进行动物基因表达研究奠定了基础。

Several pairs of PCR primers were designed and synthesized based on the coding region of porcine alpha Lactalbumin gene. The plantified 398 bp fragment coding region of porcine alpha Lactalbumin gene was amplified with recursive PCR technique. The resulting PCR products were DNA sequenced. The plantified fragment encoding region of porcine alpha-Lactalbumin gene was successfully amplified and verified by DNA sequencing. This plantified porcine alpha-Lactalbumin gene can be cloned into binary vector to produce transgenic plants.