妊娠高血压综合征患者血管紧张素Ⅱ-1型受体基因多态性的研究

Association of angiotensin Ⅱ-type 1 receptor gene polymorphism with pregnancy induced hypertension

目的 探讨血管紧张素Ⅱ 1型受体 (angiotensinⅡ type 1receptor,AT 1R)基因多态性与妊娠高血压综合征 (简称妊高征 ,PIH)的相关性。方法 采用聚合酶链反应 限制性酶切片段长度多态性 (PCR RFLP)方法检测90例妊高征患者和 90例正常妊娠妇女的AT 1R基因型 ;同时以放免法测定其外周血的血管紧张素Ⅱ (angiotensinⅡ ,AngⅡ )水平。结果 两组均存在A116 6C、C5 73T的基因多态性 ,而在 87、186、10 82和 15 17核苷酸位点均未发现基因多态性。其中妊高征组C 116 6等位基因、AC/CC基因型的频率显著高于对照组 ;5 73位点T等位基因、TT基因型的频率两组无显著性差异 ;两组AngⅡ水平无显著性差异 ;Logistic回归分析显示AT 1RA1166→C变异基因是妊高征发生的独立相关变量。结论 AT 1RA1166位点变异 (A→C)与妊高征的发生相关 ,可作为妊高征发病易感性的标记。

Objective To determine whether the gene polymorphism of the angiotensin Ⅱ-type 1 receptor (AT-1R) is associated with pregnancy induced hypertension(PIH).Methods A case-control study was carried out using 90 PIH patients and 90 normotensive pregnant women.The gene was detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis.Meanwhile,serum angiotensin Ⅱ(Ang Ⅱ) was measured by radioimmunoassay.Results There were gene polymorphisms at the nucleotides 1166 and 573 of AT1R.However,no variants were identified at the nucleotides 87,186,1082 and 1517.The frequency of C1166 allele and the AC/CC genotype of the AT-1R gene in PIH patients was significantly higher than that in the normotensive groups.There was statistically significance neither in the frenquency of T allele or TT genotype at the nucleotide 573 of AT-1R nor in the level of Ang Ⅱ among PIH and the control group.Logistic regression analysis revealed that the AT-1R A 1166 →C variant was one of the independent variables to the occurence of PIH.Conclusions The C allele is associated with PIH, which may be a marker for predisposition to PIH.