在线富集胶束电动色谱法测定唾液中的唾液酸

On-line Concentration and Determination of Sialic Acid in Saliva Using Micellar Electrokinetic Chromatography

建立了1种测定唾液酸的在线富集胶束电动色谱法。首先研究了不同的表面活性剂 (十二烷基硫酸钠、胆酸钠和脱氧胆酸钠 )、背景电解质 (磷酸盐、硼砂和3_环己胺_1_丙烷磺酸 )、缓冲溶液的 pH值及有机修饰剂 (甲醇和乙腈 )的加入对测定唾液酸的影响 ;其次研究了样品基体的组成及进样时间对富集效果的影响 ;最后考察了所建立方法的线性、检出限和重复性 ,并将该方法成功地应用于测定人体唾液中的唾液酸 。

An on-line concentration and determination of sialic acid in saliva by micellar electrokinetic chroˉmatographic method was developed.The effects of different surfactants(sodium dodecyl sulfate,sodium cholate and sodium deoxycholate),different electrolytes(sodium phosphate monobasic,sodium tetraborate and3-cyclohexylamino-1-propane sulfonic acid),buffer pH and organic modifiers(methanol and acetonitrile)on the determination of sialic acid were studied.The effects of the composition of sample matrix and injection time on the concentration efficiency of sialic acid were also studied.The calibration curve,the detection limit and the reproducibility of the developed method were presented.The method was successfully applied to the determination of sialic acid in the saliva of human body.The separation process was completed within12min.