气相色谱法直接分析生物质水解产物中的乙酰丙酸

A Gas Chromatography Method for Direct Measurement of Levulinic Acid in Biomass Decomposition Products

建立了气相色谱法直接分析生物质水解产物中乙酰丙酸的新方法。结果表明 :以苯甲酸为内标物 ,在FFAP毛细管柱上生物质水解产物有较好的分离效果 ,同时乙酰丙酸峰形较好。对同一果糖水解液测定的相对标准偏差为0.45 %(n=5) ;标准加样回收率为94.3%～100.5 %。该法具有操作简单、快速 ,结果准确。

A gas chromatography method was developed for direct measurement of levulinic acid(LA)in biomass decomposition products.With benzoic acid as internal standard,good separation was achieved with a FFAPcolumn,and the LApeak was nicely distributed as well.The relative standard deviation for five analyses of a real sample was0.45%,and the recoveries of three spiked samples ranged from94.3%to100.5%.The method developed is fastand simple to use,and provides a direct,accurate and reliable means for the determiˉnation of LA in biomass decomposition products.