表达PRRSVE基因重组质粒在小鼠诱导的体液免疫应答

Humoral immune response evicited by the recombinant plasmid expressing E gene of PRRSV in mice

利用 PCR技术对猪繁殖与呼吸综合征病毒 (PRRSV) BJ- 4株的 E基因进行修饰和改造 ,在 E基因上游加入 Kozak序列 ,扩增并克隆 E基因。将 E基因 c DNA亚克隆至真核表达载体 pc DNA3.1(+)中 ,构建了真核重组表达质粒 pc DNA- E。用pc DNA- E免疫小鼠 ,经免疫荧光抗体试验检测结果表明 ,重组质粒 pc DNA- E经 3次免疫后 ,所有小鼠血清抗体均为阳性 ,说明pc DNA- E在小鼠体内可诱导特异性的体液免疫应答反应。

By PCR, the ORF5 (E gene) of PRRS virus BJ-4 strain was modified and amplified, and sub-cloned into expression vector pcDNA3.1(+). The recombinant expression plasmid pcDNA-E was inoculated into mice. The antibodies specific for E protein in serum were confirmed by IFA. The antibody could be detected in all the mice after the third inoculation.The results showed that the recombinant plasmid pcDNA-E induced specific humoral immune responses in mice.