毛细管电泳法检测微透析液中的头孢唑林钠

In situ, in vivo, real time monitoring concentration of cefazolin sodium in rabbit plasma by microdialysis sampling and capillary electrophoresis with UV detection

微透析(MD)与毛细管电泳(CE)相结合,原位、在体、实时监测了家兔血液中头孢唑林钠(Cefazolinsodium,CFZ)浓度的变化,并初步研究了CFZ的药代动力学.结果表明,在50mmol/L的硼砂(pH9.5)缓冲液,20kV分离电压,214nm紫外检测的毛细管电泳条件下,以环丙沙星为内标,CFZ在15min之内与内标物及透析液中的其他物质达到了良好的分离.在此条件下,CFZ测定的线性范围为1～300μg/mL,检出限为0.3μg/mL(3σ),绝对检出限为2.7×10-12g.微透析灌流速度为3μL/min,探针透析率为31.8±6.8%(n=3).测定结果经NDST药动学软件分析表明,CFZ在家兔体内的清除半衰期为24.5min.

In vivo determining the concentrations of cefazolin sodium (CFZ) in rabbit plasma at different time after an i.v. dose by microdialysis sampling (MD) coupled with capillary electrophoresis (HPCE-UV) is established. The running buffer is 50 mmol/L borax buffer (pH 9.5), the running voltage is 20 kV, and the detection wave length is 214 nm. Under the optimized conditions,with ciprofloxacin as internal standard (IS), separation of CFZ from IS and other components in the dialysate is achieved within 15 min, the response of CFZ in plasma dialysate is linear over the range of 1～300 μg/mL, the detection limit is 0.3 μg/mL (3σ). Using9nL as the injection volume, the corresponding mass detection limit is 2.7×10^(-12) g. The recovery of microdialysis probe is 31.8% and RSD is 6.8%(n=3) at the perfusion rate of 3 μL/min. All data are analyzed by NDST pharmacokinetic software, the half-life for CFZ in rabbit is 24.5 min.