高效液相色谱法测定黄瓜中吡虫啉和嘧霉胺残留量

Determination of Pyrimethanil and Imidacloprid Residue in Cucumber by HPLC

本文报道了黄瓜中吡虫啉和嘧霉胺残留量高效液相色谱分析方法.黄瓜样品在乙腈中匀浆,过滤后用二氯甲烷萃取,提取残留的吡虫啉和嘧霉胺.在同一波长下,用ZORBAX,Eclipse XDB-C18液相色谱柱,以不同比例的乙腈和水进行梯度洗脱,同时分析吡虫啉和嘧霉胺.对吡虫啉和嘧霉胺添加回收率分别为82.66%～91.73%和92.76%～104.82%,最低检出限分别为0.04mg/kg和0.03mg/kg.

The determination of pyrimethanil and imidacloprid residue in cucumber by HPLC were developed. The cucumber samples were homogenized with acetonitrile, diluted with saturated NaCl water and re-extracted with dichloromethane, then the dichloromethane extracts were evaporated to dryness and residue of pyrimethanil and imidacloprid were dissolved in 5mL methanol. The methanol solutions obtained were analyzed by HPLC with HP1100 instrument fitted with ZORBAX,Eclipse XDB-C18 column. The lowest detection limit of imidacloprid and pyrimethanil were 0.04 and 0.03mg/kg, respectively, and recoveries were 82.66%~91.73% and 92.76%~104.82%, respectively.