酵母抽提物超滤制备海藻糖工艺条件的优化

Optimization of Trehalose Extracting Process by Ultrafiltration from Yeast Extract

利用响应面分析法对影响酵母抽提液中总氮截留率和海藻糖透过率的主要因素进行分析,优化超滤工艺,得到最佳超滤条件:料液质量浓度10 g／dL,料液温度42℃,操作压力0.31MPa,在最佳超滤条件下得到酵母抽提物蛋白质截留率96.5%,海藻糖透过率94.8%,所得RSA图可直观地反映各因素与蛋白氮截留率和海藻糖透过率的关系.

Response surface methodology was used to analyze the main elements influencing yeast extract' s ultrafiltration process. The optimum conditions were confirmed as follows: feed concentration 10 g/dL, temperature 42 ℃ and operation pressure 0. 31 MPa. Under this condition,the total nitrogen cut-off rate and trehalose permeation rate were 96. 5% and 94. 8%, respectively. The relationship between various factors and cut-off or permeation rates was also studied using RSA charts.