产过氧化氢酶菌株培养条件的优化

The Optimization of Culture Medium for Catalase Producer-Micrococcus lysodeikticus

通过对溶壁微球菌培养基优化,确定最佳发酵培养基,并对发酵工艺条件进行了初步的优化.最适碳源是麦芽糖,最适氮源是蛋白胨和酵母膏,最近无机盐是MnSO4;该菌株产酶的最佳培养基配方为:60 g麦芽糖,13 g蛋白胨,8 g酵母膏,1 g MnSO4,5 g NaCl,2 g NaH2 PO4·2H2O,2 g K2HPO4·3H2O,0.5 g MgSO4·7H2O,0.5 g CaCl2,1 000 mL水.从该菌株发酵过程曲线发现,该菌株大量产酶期在12-14 h.摇瓶发酵最适初始pH值为7.0,250 mL三角瓶装液量为25mL,接种体积分数为5%,培养温度为37℃.

This study mainly discussed the medium optimization for Micrococcus lysodeikticus fermentation, which can produce catalase. The results showed that the best carbon source for Micrococcus lysodeikticu fermentation is maltose, the best nitrogen source is peptone and yeast extract, and the best inorganic source is MnSO4. The optimal medium was found as follows: maltose 6%, peptone 1. 3%, yeast extract 0. 8%, NaCl 0. 5%, K2 HPO4 · 3H2 O 0. 2%, NaH2PO4 · 2H2O0.2%,MnSO40.1%,MgSO4 · 7H2O 0. 05% ,CaCl20. 05%. The fermentation curve of Micrococcus lysodeikticus showed that enzyme synthesis occurred at the time of 12-14 h. The optimal fermentation conditions were as follows: initial pH 7. 0 ;medium loading ratio of 10%(25 mL medium in 250 mL flask);seeding 5% and fermentation at 37℃for 12 h.