美味牛肝菌深层发酵条件的优化及成分分析

Study on Submerged Fermentation and Analysis of Main Components of Boletus edulis

对美味牛肝菌(Boletus edulis)深层发酵条件及菌丝体主要营养成分进行了分析研究,结果表明,最适培养基配方为:葡萄糖30.0 g,玉米粉20.0 g,酵母膏7.0 g,KH2PO4 2.0 g,MgSO4·7H2O 1.0 g,VB1 0.01 mg,CaCO3 2.0 g;最优发酵条件的初始pH值5.2-5.8,振荡速度180 r／min,培养温度27℃,100 mL三角瓶装液量60 mL.并对美味牛肝菌深层培养菌丝体中蛋白质成分、灰分、脂肪、粗纤维、矿物质及多糖等进行了测定和分析.

This paper explores the suitable fermentation conditions affecting the mycelia growth of Boletus edulis. Experimental results indicated that the optimum composition of the fermentation medium is as following: glucose 30. 0 g, Corn meal 20. 0 g,yeast extract 7. 0 g, KH2PO42. 0 g, MgSO4 ·7H2O 1. 0 g, VB1 0. 01 mg and CaCO3 2. 0 g. The highest yield of mycelia was obtained under the conditions when the shaker rotation speed, pH, temperature and medium loading amount were controlled at 180 r/min, 5. 2-5. 8,27 ℃ ,and 60 mL/100 mL,respectively.