纤维素酶Celluclast1.5L在酵母菌原生质体制备及再生中的应用

The Use of Enzyme Celluclast 1. 5 L in Production and Regeneration of Yeast Protoplast

比较了在不同的酶浓度、处理时间、渗透剂和pH值下纤维素酶Celluclast 1.5 L和蜗牛酶在酵母菌原生质体的形成和再生中的应用.在最佳条件下,单独使用纤维素酶的原生质体的形成率可达60%左右,原生质体的再生率可达10%以上,与蜗牛酶类似.

This paper indicated the use of enzyme Celluclast 1. 5 L (a liquid cellulase preparation) in production and regeneration of protoplast of yeast. Under the optimum conditions , the production yield of yeast protoplast is about 60% and the regeneration yield of yeast protoplast is above 10%.