生长素结合蛋白基因在黄瓜果实发育中的功能研究

FUNCTION OF AUXIN-BINDING PROTEIN GENE DURING CUCUMBER FRUIT DEVELOPMENT

应用逆转录-聚合酶链式反应(RT-PCR),从黄瓜子房(幼果)中扩增出生长素结合蛋白(ABPl)cDNA片段。该基因在开花前1天的子房中表达信号较弱,在授粉后2、4和6天的幼果中表达较强;在开花后2天有单性结实能力的子房中表达信号较强,不能形成果实的子房中信号较弱,所以ABPl基因可能参与黄瓜果实的生长发育过程。将拟南ABPl芥基因转入黄瓜中,转基因黄瓜的单性结实率平均为31.7%,高于对照(19.9%)。由于黄瓜的单性结实主要与生长素有关,所以,转基因植株单性结实率的提高可能是由于子房增强了对自身所含生长素的敏感性所致,说明生长素结合蛋白参与生长素在黄瓜果实生长发育中的生理作用。

A eDNA fragment that encodes auxin-binding protein 1 was amplified by reverse polymerase chain reaction fi-om ovary of cucumber.Its expression signals were weak in the ovary of 1 d before anthesis,while got strong in 2,4 and 6 d after pollination.Among the unpollinated ovary of 2 d after anthesis,those that got enlarged had strong expression signals;the others that were wilting had weak signals.This indicated that auxin-binding protein 1 gene possibly play a role in cucumber fruit development.When auxin-binding protein 1 gene of Arabidopsis was trans- formed into cucumber,the parthenocarpic rate of transgenic plants was 31.7%,higher than the control.This result showed that the sensibility to auxin was increased in transgenic plants.