电针对士的宁诱发大鼠脊髓背角c-fos基因表达的影响(英文)

Effect of electron-acupuncture on c-fos gene expression in posterior horn of spinal cord in rats induced by strychnine

背景:大鼠皮下注射士的宁后电针解溪或昆仑穴,是否可诱发出脊髓跨节段的c-fos表达。目的:通过电针对士的宁诱发大鼠脊髓背角c-fos基因表达情况,探讨针刺信息传递中脊髓兴奋性的作用。设计:完全随机对照研究。单位:南京中医药大学江苏省针灸学重点实验室。对象:采用健康雄性Wistar大鼠,体质量200～230g。随机分为空白对照组,假电针组,士的宁组,电针解溪组,士的宁解溪组,电针昆仑组和士的宁昆仑组等7组,每组8只。干预:空白对照组:不做任何处理;假电针组:臀部皮下注射生理盐水(与士的宁等容量,下同)30min后解溪穴插针不给电刺激;士的宁组:臀部皮下注射士的宁(1.0mg/kg,下同)30min后解溪穴插针不给电刺激;电针解溪组:注射生理盐水30min后电针解溪穴;士的宁解溪组:注射士的宁30min后电针解溪穴;电针昆仑组:注射生理盐水30min后电针昆仑穴;士的宁昆仑组:注射士的宁30min后电针昆仑穴。主要观察指标:各组大鼠不同脊髓节段背角的c-fos表达。结果:皮下注射士的宁可在大鼠脊髓L5诱发中等量的c-fos表达,而皮下注射士的宁后电针解溪或昆仑穴,不仅可使脊髓L5的c-fos表达增强,同时在T12和C8节段也可诱发c-fos表达;其标记最密集的区域主要在背角的Ⅱ,Ⅲ,Ⅳ层亦有少量分布。结论:在脊髓兴奋性提高的条件下,针刺信息可能?

BACKGROUND:Whether c-fos expression in trans-segments of spinal cord is induced or not with electron-acupuncture on Jeixi(ST 41)or Kunlun(BL 60) after strychnine s.c in rats. OBJECTIVE:To probe into the mechanism of spinal cord excitation during needling information transfer influenced by electron-acupuncture on c-fos genetic expression in posterior horn of spinal cord induced with strychnine. DESIGN: A completely, randomized and controlled study. SETTING:Key Laboratory of Acupuncture of Jiangsu Province, Nanjing University of Traditional Chinese Medicine. PARTICIPANTS:Normal male Wistar rats were employed,weighing from 200 g to 230 g in the experiment.The rats were randomized into 7 groups,named as blank control group,pseudo-electric-needling group,strychnine group, electric-needling Jeixi group,strychnine-Jeixi group, electric-needling Kunlun group and strychnine-Kunlun group,8 rats in each group. INTERVENTIONS:Blank control group:No any management was done.Pseudo-electric-needling group:Normal saline was s.c on buttocks(same volume as strychnine,to be same in other groups).Thirty minutes later,needling was done on Jeixi(ST 41) without electric stimulation.Strychnine group:Strychnine was s.c on buttocks(1.0 mg/kg, to be same in other groups).Thirty minutes later,needling was done on Jeixi (ST 41) without electric stimulation.Electric-needling group:Electron-acupuncture was given on Jeixi (ST 41) 30 minutes after s.c saline normal.Strychnine-Jeixi group:Electron-acupuncture was given on Jeixi (ST 41) 30 minutes after strychnine.Electric-needling Kunlun group:Electron-acupuncture was given on Kunlun(BL 60) 30 minutes after normal saline.Strychnine-Kunlun group:Electron-acupuncture was given on Kunlun (BL 60) 30 minutes after strychnine s.c. MAIN OUTCOME MEASURES:c-fos expression in posterior horn of different spinal segments in rats in every group. RESULTS:c-fos expressions of moderate amount were induced in L6 segment of spinal cord in rats after injection with strychnine.But by electron-acupuncture on Jeixi (ST 41) or Kunlun(BL 60) after subcutaneous injection with strychnine,c-fos expression was strengthened not only in L5 segment of spinal cord,but also in T12 and C2 segments simultaneously.The densest labeled regions were chiefly distributed in Ⅱand Ⅲlayers,and also in Ⅳlayers of posterior horn in few amount. CONCLUSION:With the improvement of spinal cord excitation, the needling information transmits probably in trans-segments along spinal cord. It is suggested that the change in spinal cord excitation is probably one of the important bases affecting needling-information transfer.