乳腺癌组织中耐药和转移相关基因联合表达的临床病理分析

Pathological analysis of drug resistance and metastasis-associated genes in breast cancer

目的 :联合检测乳腺癌组织中 P-糖蛋白 (P-gp)、谷胱甘肽 -S-转移酶 (GST-π)、拓朴异构酶 (Topo ) 3种耐药基因和 nm2 3、CD44 V62种转移相关基因 ,观察其表达情况与临床病理特征的关系。方法 :采用 S-P法和二步法的免疫组化方法检测耐药基因 P-gp、GST-π、Topo 和肿瘤转移相关基因 nm2 3 -H1、CD44 V6在 112例乳腺癌中的表达。结果 :P-gp、GST-π、Topo 与nm2 3、CD44 V6的阳性表达率分别为 5 6.2 %、67.8%、72 .3 %和 65 .2 %、72 .3 % ,相互之间过表达相关性不明显 (P>0 .0 5 ) ;nm2 3、CD44 V6与淋巴结转移有关 (P<0 .0 1)。结论 :作用于乳腺癌的耐药基因和肿瘤转移相关基因相互之间无相关性 ,可能具有不同作用机制 ,每个基因具有独立的临床意义。

Objective:To detect the expression of 3 kinds of drug resistance genes:P-gp、GST-π、TopoⅡ and 2 kinds of metastasis-associated genes:nm23、CD44V6 in breast cancer,and to investigate their expressions and relations and clinical associations.Methods:Detect the expressions of drug resistant genes:P-gp、GST-π、TopoⅡ metastasis-associated genes-nm23/CD44V6 in 112 breast cancer patients by immunohistochemistry.Results:The positive ratio of P-gp、GST-π、TopoⅡ、nm23、CD44V6 was 56.2%、67.8%、72.3% and 65.2%、72.3% respectively.Their interaction was not distinct,nm23、CD44V6 were associated with lymph node metastasis.Conclusion:Drug resistant genes and metastasis-associated genes have no relation in breast cancer,probably have different working mechanism and independent significance.