浙江省肾综合征出血热灭活疫苗的研究

Study on Inactive Vaccine Against Hemmorrhagic Fever with Renal Syndrome in Zhejiang Province

目的 通过对分离到的汉坦病毒 (HV)免疫原性及病毒滴度的选育 ,选择出疫苗毒株及筛选出不破坏病毒血凝抗原的灭活剂和疫苗基质细胞 ,提高疫苗的规模化生产技术 ,生产出副反应轻 ,免疫原性和现场防病效果好的各种剂型的疫苗 ,满足人群免疫预防的需要。方法 分离到的HV株 ,应用多种方法进行免疫原性和动物保护试验选育 ;疫苗灭活剂筛选通过与甲醛灭活效果比较 ,观察对血凝素抗原的影响 ,以此作为判断指标 ;疫苗基质细胞建立主要通过观察病毒在各种细胞上的增殖滴度、基质细胞的贴壁时间及收液次数等方法 ,选择基质细胞。结果 从 45株病毒株中成功选育出Z10 (汉滩型 )和Z3 7(汉城型 )疫苗病毒株 ,两株疫苗免疫原性好、病毒滴度高 ;成功选择出 β 丙内酯作为疫苗灭活剂 ,它不破坏病毒的血凝素抗原 ,接种反应轻 ;建立的长爪沙鼠肾原代细胞作为疫苗基质 ,不仅病毒滴度高 ,而且贴壁效果好 ,可以做到多次收液的目的。结论 我们研制的疫苗无论是单价的Ⅰ型、Ⅱ型疫苗 ,还是双价 (Ⅰ型和Ⅱ型混合 )疫苗 ,接种人体后副反应轻 ,免疫效果好 ,现场防病效果也很理想 ,是目前控制肾综合征出血热流行最有效和经济的手段和措施 ,值得大力推广。

Objective To select the vaccine strain of hantanvirus and the cell for inculation,the immunogenicity of hantanvirus and virus titer are tested.To establish the technics of vaccine development, the vaccine,the inactived adjuvant that do not weaken the hemoglutinin of hantanvirus is selected. To develop vaccine which has low aside effect and good immunogencity preventing clinical infection of hemmorrhagic fever with renal syndrome(HFRS). Methods By immunogenicity and pretentive effect test of animal,the vaccine strain is selected from isolates of hantanvirus;In comparison with formalin,the inactive effect is observed and used as guideline;To select the cell for vaccine development,the virus titer in cell,the time that cell welt and the times of harvest are observed. Results The Z10 strain (hantaan virus) and Z37 strain (Seoul virus) of hantanvirus is selected as vaccine strain from 45 strains.Both of them have good immunogenicity and high virus titer; βpropiolactone as inactived adjuvant does not weaken the hemagglutination antigen of vaccine and have low aside effect; The primary cell of merones unguiculatus kidney tissue as virus inculation cell have high virus titer and welt well. It can also havest virus many times. Conclusion Momovalent inactive vaccine (type Ⅰ,Ⅱ)and duvalent inactive vaccine against HFRS which has been developed by Zhejiang province center for disease control and preventive have low aside effect and good immunity. All of them can can protect clinical infection of HFRS and is worthwhile to used.