氯化汞对小鼠睾丸生殖细胞的DNA损伤作用

Effects of mercuric chloride on DNA damage ofspermatogenic cells of mice testicle

探索氯化汞对雄性小鼠生殖细胞毒性的分子遗传机制。分别以 0 .0 1、0 .10、1.0 0 mmol/L的氯化汞体外处理小鼠睾丸细胞和以 0 .5、1.0、5 .0 μmol/kg的氯化汞体内暴露小鼠 ,应用单细胞凝胶电泳技术检测生殖细胞 DNA的损伤。体外处理 3种剂量组小鼠睾丸生殖细胞 DNA损伤率显著高于阴性对照组 ( 0 mmol/L组 ,P<0 .0 0 1) ;0 .1、1.0 mmol/L剂量组慧星细胞迁移率显著高于阴性对照组 ( P<0 .0 0 1,P<0 .0 5 )。体内暴露 3种浓度氯化汞组小鼠睾丸生殖细胞 DNA损伤率显著高于阴性对照组 ( 0μmol/kg,P<0 .0 0 1) ,5 .0 μmol/kg组慧星迁移率显著高于阴性对照组 ( P<0 .0 5 )。一定剂量的氯化汞处理引起生殖细胞 DNA损伤作用可能是氯化汞细胞毒性的机制之一。

To study the molecular genetic mechanism of mercuric chloride on spermatogenic cell damage, isolated spermatogenic cells of mice testicle were exposed to mercuric chloride (respective concentration: 0.01、0.1、1.0 mmol/L)and male mice were administered by mercuric chloride (0.5、1.0、5.0 μmol/kg) in the abdominal cavity. The proportion of DNA damage and length of DNA migrations of spermatogenic cell were determined with single cell gel electrophoresis(SCGE). The DNA damage rate(0.01、0.1、1.0 mmol/L group and 0.5、1.0、5.0 μmol/kg group) and length of DNA migrations(0.1、1.0 mmol/L group and 5.0 μmol/kg group) of spermatogenic cell of mice testicle treated with mercuric chloride in vitro and vivo was higher that of normal group(P<0.001,P<0.05). Effects of mercuric chloride on DNA damage of spermatogenic cell were possible mechanism of mercuric chloride on spermatogenic cell damage.