神经干细胞脑内移植后的存活、迁移和分化

Survival,Migration and Differentiation of Neural Stem Cells After Transplantation into the Brain

从胚胎或成体大鼠脑组织、人胚脑组织均能分离到神经干细胞 ,将它们进行体外原代培养扩增或永生化后植入脑内 ,均能观察到其在脑内的迁移和分化现象。其分化能力主要取决于移植部位的脑内微环境 ,但这种影响作用是相对的。同时 ,体外培养环境如培养时间和细胞融合程度、维甲酸类诱导分化剂处理、NGF转导处理再移植或与嗜铬细胞 (分泌NGF)共移植等 ,也能决定神经干细胞脑内移植后向神经元方向分化的能力。神经干细胞移植为中枢神经系统功能重建和神经再生带来新的希望。

Neural stem cells (NSC) have been successfully isolated from embryonic or adult rat brain,as well as from embryonic human brain.NSC can be expanded epigenetically in vitro as primary cultures or immortalized genetically for large-scale production.They could migrate,integrate into the host brain and differentiate into mature neurons after transplantation into the animal brain.The potential of NSC differentiation is mainly dependent upon the microenvironment in the brain where they are grafted.However,other factors,including the in vitro culture condition such as periods of culture time,level of cell confluence,treatment with retinoic acid,transduction with NGF gene,and the co-transplantation of chromaffin cell,also play roles in deciding on their capability of differentiation.The transplantation of neural stem cells can provide a powerful way for the functional rebuilding and regeneration in the CNS.;