太空环境改变生物工程细胞CHO(dhfr^-)的生长特性

Effects of Spaceflight on CHO(dhfr-)Cells Growth Characteristics

目的 :了解太空诱变对生物工程细胞CHO(dhfr-)生长特性的影响。方法 :将CHO(dhfr-)生物工程细胞株搭载于我国第 1 8颗返回式卫星 ,经 1 8天太空飞行 ,回收后对存活细胞进行单克隆化 ,获得 1 5 9株单克隆细胞。选取生长快、慢不同的 3株克隆 ,利用光镜、MTT法、FCM分析及3 H掺入法观察细胞生长特性。结果 :经太空诱变后 ,CHO(dhfr-)细胞呈多种形态 ;G1期细胞显著增多 ;生长速度和大分子生物合成呈现多向性变化 ,无特定规律性。结论 :太空环境可影响生物工程细胞CHO(dhfr-)的生长特性 ,为进一步筛选优化的生物工程细胞提供了可能。

Objective: To investigate the effects of spaceflight on CHO(dhfr-) cells growth characteristics Methods: CHO(dhfr-) cells were loaded and flight 18days on the No.18 recovered satellite. All survival cells were monocoloned and 159 cell lines were obtained. 3 cell lines selected randomly were used as cell models, the experimental methods such as cell morphological observation, MTT assay, FACS and 3H incorporation were used for analysis of cell growth characteristics. Results: Comparing with the control group, CHO(dhfr-) cells appeared multiple cell morphological changes and the cell number of G1 phase was increased markedly (p<0.05). Growth rate ,biosynthesis of nucleic acid and protein in mutated cells were varied without specifically disciplinarian. Conclusion: The data suggested that effects of spaceflight on growth characteristics were complicated and multiplex in CHO(dhfr-) cells.;