热休克蛋白70(HSP70)基因在中国对虾染色体上的定位

Localization of gene encoding heat shock protein, HSP70,to chromosome of Chinese shrimp Fenneropenaeus chinensis

以热休克蛋白70(HSP70)基因为探针,利用荧光原位杂交(FISH)的方法,将其初步定位在中国对虾(Fennerope-naeus chinesis)染色体上。观察150组精巢细胞染色体,有111组染色体有3个杂交信号,占所观察的74％,由此得出,热休克蛋白70基因(HSP70)很可能存在于中国对虾减数分裂细胞3对染色体的3个位点上。本研究首次将功能基因定位在了对虾染色体上,为中国对虾的遗传物理图谱的构建提供了有效可行的方法。

A conservative gene, heat shock protein 70( HSP70), was mapped to the chromosomes of the Chinese shrimp ( Fenneropenaeus chinensis ) with Fluorescence in situ hybridization ( FISH) . FISH is a powerful tool for chromosome assignment and analysis, and it has been successfully applied in many species. However, this is stili a challenge in the Chinese shrimp( Fenneropenaeus chinensis) :one reason is the large chromosome number of F. chinensi(2n= 88); the other reason is that the chromosomes of F. chinensi are compressed with the similar figure.In this study, the HSP70 was firstly mapped to the chromosome of the Chinese shrimp (Fenneropenaeus chinensis ) . With the successive chromosome preparation, probe construction and fluorescence in situ hybridization, HSP70 produced signals on nuclei and metaphase haploid. Analysis of chromosomes shows that 111 of 150 (74%) observed sets of testis haploid chromosomes produced three hybridization signals, which located respectively on three different chromosomes. The results indicated that HSP70 was assigned to three loci of the haploid chromosome. The FISH result suggests that HSP70 may have three loci haploid cells of Chinese shrimp. This study is the first step for the chromosome assignment of many interesting genes, which may provide important insight on the organization and evolution of the Crustacea genome. In addition, the chromosome assignment of the genes could promote the cytogenetical analysis and the physical map of F. chinensi.