摘要
白介素-2(IL-2)广泛用于治疗恶性肿瘤,由于大剂量可引起严重的低血压且机制不明,限制了其大剂量的使用。本研究用国产重组人白介素-2(rhIL-2)复制大鼠低血压模型并探讨其机制。24只wistar大鼠随机分成3组(每组n=8):正常对照组,IL-2实验组(rhIL-2)和氨基胍(AG)治疗组。结果显示:(1)IL-2可使大鼠提睾肌微动脉扩张及MAP下降,肺、肾、肝组织Evan′sBlue含量明显增加。(2)AG可使rhIL-2引起的低血压回升及微动脉缩小,肺组织Evan′sBlue含量明显下降。提示:rhIL-2引起低血压,可能与IL-2诱导NO产生,使血管扩张及通透性增加有关。
? It was reported that hypotension induced by IL-2 in cancer therapy was associated with vascular leak syndrome.(VLS) or vascular dilatation mediated by nitric oxide (NO).This study aimed at investigating whether recombinant human IL-2(rhIL-2)could induce hypotension in rats and whether both VLS and NO were involved in hypotension induced by rhIL-2.24 wistar rats were randomly divided into 3 groups (1) control (NS),(2)rhIL-2,(3)rhIL-2 pluse aminoquanidine (AG).The results showed that:(1)In contrast to the control group,the arterioles were also dilated and MAP decreased significantly in rhIL-2 group.The content of Evan′s Blue was greatly increased in the tissue of lung,kidney and liver in rats at 180min.(2)In contrast to rhIL-2 group,MAP and the arterioles diameter were significantly ameliorated in rhIL-2+AG group.The content of Evan′s Blue in the tissue of lung was significantly reduced.Conclusion:Hypotension induced by rhIL-2 is attributed to both the dilatation of the arterioles and the increment of the vascular permeability and its mechanism may be related to the increment of inducible nitric oxide synthesis.
出处
《中国实验动物学报》
CAS
CSCD
1998年第1期12-18,共7页
Acta Laboratorium Animalis Scientia Sinica