糖尿病神经病变血清抗神经节苷脂抗体的观察

Measurement of anti-GS-ab in diabetic polyneuropathy patients

目的 了解 2型糖尿病周围神经病变 (DPN)免疫功能状况。方法 受试者分为糖尿病无神经病变组 (DM组 )、DPN组、正常对照组 (N组 ) ,每组 30人。采用美国NicoletVikingⅣ型肌电图仪测定神经传导速度 ,固相酶免法测定各组血清抗神经节苷脂抗体 (anti GS ab)中的anti GS IgM ab、anti GS IgG ab ,同时观察部分细胞免疫及体液免疫及自身抗体指标。 结果 DPN组较其它两组体液免疫功能增高 ,IgM及自身抗体亦明显升高。anti GS ab水平明显高于其它两组 ,T淋巴细胞亚群CD4+ 、CD8+ 、IL 2值水平明显下降 ,IL 1β、TNF α显著增高。逐步回归分析 ,DPN组对anti GS ab有显著作用的因素依次为DPN的临床分级 (DPNC)、CIC、IL 1β。 结论 DPN患者体内确存在着免疫功能紊乱 ,了解DPN自身免疫状况 ,对DPN的早期诊断、病情判断及治疗具有重要意义。

Objective To investigate the relationship between anti-GS-ab and cell immune in diabetic polyneuropathy (DPN).Methods There were NIDDM patients without DPN(group DM), NIDDM with DPN(group DPN), health people(group N). Each group had 30 people. We used American Nicolet Viking Ⅳ electromyography to measure neural conduction rate and immobilized enzyme immunoassay to measure anti-GS IgM-ab 、 anti-GS IgG-ab, and to observe cellular immunity,humoral immunity,autoimmunity antibody.Results In group DPN, anti-GS-ab level was higher than that in group N and group DM,T-lymphocyte subgroup CD 4+ , CD 8+ ,IL-2 level obviously decreased, IL-1β,TNF-α significantly increased. Using regulation analysis, in sequence.Conclusion There are immune function chaos in patient's body.To understand autoimmunity condition is valuable to early diagnosis of DPN, judgment of clinical condition, remedy effect.