膀胱癌患者外周血特异性标记物CK—20 mRNA检测及临床意义(英文)

CK-20 mRNA expression in peripheral venous blood as specific tumor marker in bladder transitional cell carcinoma

目的膀胱癌患者外周血中特异性角蛋白上皮CK—20mRNA在诊断中的意义。方法以半巢式RT-PCR检测外周血有核细胞成分中CK—20mRNA。结果91例膀胱癌中检出CK—20mRNA 37例,阳性率41%,其中证实有转移的20例中17例阳性(85%),25例健康对照组均为阴性。外周血细胞CK—20mRNA的阳性率在远处转移的病例中显著升高(P<0.01)。结论检测膀胱癌患者外周血CK—20mRNA对早期发现膀胱癌细胞的血行播散有重要参考价值。

s: Objective: A sensitive method was attempted to detect the specific epithelial keratin CK-20 mRNA in peripheral venous blood from patients with bladder carcinoma. Methods: Reverse transcription coupled with two-step polymerase chain reaction (Nested RT-PCR) was used to detect CK-20 expression in the peripheral blood. Results: Detection of CK-20 expression was positive in 37 of 91 patients with bladder carcinoma (41%). Among 20 patients with distant metastasis, 17 were positive (85%). CK-20 was not detected in the blood samples from 25 normal individuals. The frequency of positive CK-20mRNA expression was signficantly higher in those with distant metastasis. Conclusion: The presence of CK-20mRNA expression in peripheral blood may be used as an early indicator of hematogenous metastasis of bladder carcinoma cells.