200株酵母菌对氟康唑的耐药性分析

Study on resistance of yeast fungus in clinical sample to fluconazol

目的检测酵母菌临床分离株对氟康唑的耐药状况。方法收集临床酵母菌分离株,采用2003年美国临床实验室标准化委员会(NCCLS)推荐的酵母菌纸片扩散敏感试验(M44-P)。结果在所分离到的200株酵母菌中,86.5%分离自呼吸道标本,共9个菌种,其中三分之二为白色念珠菌,其余依次为热带念珠菌、克柔念珠菌和光滑念珠菌等。分离到的8株克柔念珠菌全部耐药,而构成比最高的白色念珠菌和热带念珠菌的平均耐药率低于10%。结论白色念珠菌和热带念珠菌使我院现阶段主要的病原酵母菌,主要来源于呼吸道标本。所检测酵母菌对氟康唑的总体耐药率不高,提示氟康唑目前仍是有效的抗酵母菌感染的药物。

Objective: To investingate the resistance of yeast fungus in clinical sample to fluconazol. Methods: yeast fungus were isolated and identified from clinical sample, and susceptibility was test with K-B method recommended by NCCLS in 2003. Results: More than 86% of pathogenic yeast fungus were isolated from samples of respiratory tact, There were nine species yeast fungus were isolated from clinical sampl,and near two third of them were the Candida albians, and followed by the Candida tropicalis and Candida glabrata etc. The resistant rate of Candida kusei to fluconazol reached 100%, but the average resistant rate of Candida albicans and Candida trooicalis were lowere than 10%. Conclusions: Candida albicans and Candida tropicalis are the most comm.on pathogenic yeast funguns, and most of them originate from respiratory tract. The resistan rate of tested pathogens is low overall, and it suggests that the fluconazol is the effective antifungal drugs in our hospital at present.