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番茄Tm-2^2基因是一个受外源乙烯调节的抗病基因 被引量：7

Tomato Tm-22 Is a Resistance Gene by Regulated Exo-ethylene Stimulation
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摘要 Tm-22基因编码一CC-NBS-LRR抗病蛋白,Tm-22植株的抗病毒反应表现为系统性坏死症状。试验结果表明,Tm-22基因转化体种胚下胚轴的伸长受到外源乙烯的抑制,乙烯的持续性刺激能够诱导H2O2的产生和氧离子自由基(ROS)的猝发,以及PR-1a基因mRNA的转录。可以初步认为Tm-22转化体的抗ToMV反应是一个与乙烯信号转导途径有关的反应。 Tomato Tm-2~2 gene encodes a resistance protein with a CC-NBS-LRR structure,which gives resistant appearance with systemic necrosis in anti-virus response.The experimental investigation demonstrated that continuous exo-ethylene stimulation inhibited hypocotyl elongation and induced H_2O_2 generation,reactive oxidative species(ROS) burst and transcription of the PR-1a gene.The results suggested that the anti-virus activity of Tm-2~2 transformants was associated with the ethylene signaling pathway.
作者 姜国勇 杨仁崔
机构地区 莱阳农学院 福建农林大学
出处 《病毒学报》 CAS CSCD 北大核心 2004年第4期359-363,共5页 Chinese Journal of Virology
基金 国家自然基金项目资助(30170633)
关键词 Α基因 坏死 性刺激 PR ROS 抗病毒 基因编码 外源 番茄 抗病基因 Tm-2~2 gene ethylene tomato transgenic tobacco
分类号 Q945 [生物学—植物学] S831.5 [农业科学—畜牧学]
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参考文献10

  • 1Hall T J.Resistance at the Tm-2 locus in the tomato to tomato mosaic virus[J].Euphytica,1980,29:189-197.
  • 2Lanfermeijer F C,Dijkhuis J,Sturre M J G,et al.Cloning and Characterization of the Durable Tomato Mosaic Virus Resistance Gene Tm-22 from Lycopersicon esculentum[J].Plant Mol Biol,2003,52:1037-1049.
  • 3Jong D,Yakimova E T, Kapchina V M,et al.A critical role for ethylene in hydrogen peroxide release during programmed cell death in tomato suspension cells[J].Planta,2002,214(4):537-545.
  • 4Thordal-Christensen H,Zhang Z,Weiy,et al.Subcellular localization of H2O2 in plants.H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction[J].Plant J,1997,11:1187-1194.
  • 5Van der Hoorn R A,Laurent F,Roth R,et al.Agroinfiltration is a versatile tool that facilitates comparative analyses of Avr9/Cf-9-induced and Avr4/Cf-4-induced necrosis[J].Mol Plant Microbe Interact,2000,13(4):439-446.
  • 6Cornelissen B J,Horowitz J,van Kan J A,et al.Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group[J].Nucleic Acids Res,1987,15(17):6799-6811.
  • 7Ohme-Takagi M,Suzuki K,Shinshi H.Regulation of ethylene-induced transcription of defense genes[J].Plant Cell Physiol,2000,41(11):1187-1192.
  • 8Weber H,Schultze S,Pfitzner A J.Two amino acid substitutions in the tomato mosaic virus 30-kilodalton movement protein confer the ability to overcome the Tm-22 resistance gene in the tomato [J].J Virol,1993,67(11):6432-6438.
  • 9Doke N,Miura Y,Sanchez L M,et al.The oxidative burst protects plants against pathogen attack:mechanism and role as an emergency signal for plant bio-defense--a review [J].Gene,1996,179:45-51.
  • 10Heath M C.Hypersensitive response-related death[J].Plant Mol Biol,2000,44(3):321-334.

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病毒学报
2004年 第4期

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