抗前列腺干细胞抗原单克隆抗体的制备及初步鉴定

The preparation and identification of monoclonal antibody against prostate stem cell antigen

目的 制备抗前列腺干细胞抗原 (PSCA)单克隆抗体 (McAb) ,并对其特性进行鉴定。方法 应用PC 3细胞免疫BALB/c小鼠 ,按常规方法融合并经间接ELISA方法筛选 ,制备稳定的抗PSCAMcAb ,并对McAb的亚型、组织特异性进行鉴定。结果 制备出抗PSCA单克隆抗体 ,免疫扩散鉴定为IgG1亚类 ,ELISA法测定其腹水效价为 5× 1 0 -5。组织学检查与前列腺癌组织呈阳性反应 ,与其他肿瘤组织均呈阴性反应 ,和人体正常组织细胞无一出现阳性反应。结论 初步制备出一株抗PSCA的单克隆抗体细胞株 ,能特异性识别前列腺癌组织 ,对前列腺癌的诊断和治疗提供有用的工具。

Objective To prepare and identify monoclonal antibody against prostate stem cell antigen(PSCA).Methods BALB/c mice were immunized by PC-3 cell line.After fusing and screening,the anti-PSCA McAb's characterizations were determined.Results A marine McAb was produced,which raised against PSCA,belonged to IgG1 subclass.The McAb demonstrated positive reaction with prostate cancer,but negative reaction with non-prostate cancer tumor samples.As far as normal human tissue is concerned,no positive reaction happened.Conclusion One hybridoma secreting specific monoclonal antibody against PSCA is preliminarily established,which could be used in targeting diagnosis and treatment of prostate cancer.