内源性哇巴因在肾小球疾病伴高血压及肾功能减退时的变化

Variance of endogenous ouabain in glomerulonephropathy with hypertension and renal hypofunction

目的 :研究内源性哇巴因 (EO)在肾小球疾病伴高血压及肾功能减退时的变化 .方法 :采用酶标免疫吸附抗原包被法检测正常对照组和肾小球疾病伴高血压及肾功能减退组患者的血清EO浓度 ,观察EO与高血压及肾功能减退的关系 .结果 :各种肾小球疾病 ,无论血压增高与否 ,患者均有血清EO浓度的升高 (P <0 .0 1)肾小球疾病肾功能正常组(1.4 6± 0 .5 4 ) μg/L、氮质血症组 (2 .74± 0 .6 3) μg/L及尿毒症组 (3.5 8± 0 .79) μg/L的血清EO水平均明显高于正常对照组(0 .87± 0 .35 ) μg/L (P <0 .0 1) .且随肾功能的减退 ,血清EO水平呈增高趋势 .与正常对照组相比 ,无论血清或尿的 β2 微球蛋白 (β2 MG)增高与否 ,其血清EO水平都高于正常 ,表明在肾小球发生病变后 ,即使目前反应肾小球及肾小管功能最敏感的指标之一还没有发生变化 ,血清EO已经升高 .结论 :血清EO在肾小球肾炎伴高血压及肾功能减退时明显升高 .血清EO反应肾功能损害比血、尿 β2 MG更敏感 .

AIM: To study the variance of endogenous ouabain (EO) in glomerulonephropathy with hypertension and renal hypofunction. METHODS: The serum EO of normal controls and patients with glomerulonephropathy accompanied with hypertension and renal hypofunction was detected with a method of ELISA coating, to find the relationship between the EO concentration and renal hypofunction. RESULTS: Patients with glomerular diseases had an increased serum EO concentration, which was not related to blood pressure (P<0.01). The EO levels (μg/L) in group of normal renal function (1.46±0.54),azotemia (2.74± 0.63) and uremia (3.58±0.79),were all higher than that of normal controls (0.87±0.35) (P<0.01). A positive correlation was found between the degree of renal disfunction and serum EO concentration. Serum EO concentration changed earlier than serum and urineβ 2-MG. CONCLUSION: The level of EO increases obviously in glomerulonephritis especially with hypertension and renal hypofunction. Serum EO is more sensitive than blood and urine β 2-MG in detection of renal dysfunction.