荷移分光光度法测定克拉霉素

Spectrophotometric determination of clarithromycin based on charge transfer reaction between clarithromycin and alizarin

目的 :建立测定制剂中克拉霉素含量的方法 .方法 :利用克拉霉素与茜素在水 醇介质中发生电荷转移反应 ,形成电荷转移络合物 ,采用可见分光光度法测定 .结果 :荷移反应生成的荷移络合物在 5 4 6nm处有最大吸收 ,络合物的组成比是 1∶1,稳定常数是 3.4× 10 4,表观摩尔吸光系数 7.31× 10 3 L/(mol·cm) ,药物浓度在 1～ 10 0mg/L范围内服从比耳定律 ,方法平均回收率在 98.0 %以上 ,克拉霉素浓度为 5 0mg/L时 ,相对标准偏差 (RSD)为 1.30 % (n =6 ) .结论 :方法稳定、准确、灵敏、快速 ,对样品的测定结果令人满意 .

AIM: To establish a method for rapidly determinating the content of clarithromycin in preparation. METHODS: To utilize visible spectrophotometry based on the charge transfer reaction between clarithromycin as donor and alizarin as acceptor in alcohol-water solution. RESULTS: The apparent molar absorptivity of complex was 7.31×10 3 L/(mol·cm) at 546 nm, while the composition ratio and stability constant of charge transfer complex were 1∶1 and 3.4×10 4 respectively. Beer’s law was strictly obeyed in the range of 1-100 mg/L of clarithromycin. The average recovery was over 98.0%. The average standard deviation of six determinations was 1.30% when the concentration of clarithromycin was 50 mg/L. CONCLUSION: The proposed method is stable, accurate, sensitive and rapid, which can contribute to satisfactory results for the determination of clarithromycin in preparation.