天津市支气管哮喘病人血管紧张素转移酶基因多态性研究

Polymorphism of angiotensin-converting enzyme gene and susceptibility to patients of asthma in Tianjin

目的 探讨血管紧张素转移酶 (ACE)基因插入 (insertion,I)与缺失 (deletion,D)多态性与支气管哮喘易感性的关系。方法 采用聚合酶链反应 (PCR)方法检测 18例哮喘患者 ,15名正常健康对照者的 ACE基因型。结果 哮喘组 ACE基因中 DD基因型频率和 D等位基因频率分别为 6 1%和 72 % ,而正常对照组为 2 0 %和37% ,两组比较差异有统计学意义 (P<0 .0 5 )。结论 提示 ACE基因 DD等位基因型与哮喘的遗传易感性相关 ,可能是哮喘的危险因素之一。

Objective Angiotensin-converting enzyme (ACE) plays a key role in the metabolism of angiotensin Ⅱ (AT-Ⅱ) and inactivation of bradykinins and tachykinins,which are potent bronchial constrictors and mediators of inflammatory asthma,and ACE is heavily expressed in the lungs.An insertion-deletion (D/I) polymorphism of ACE gene has been shown to be associated with the levels of ACE.Whether or not the polymorphism of ACE gene is associated with asthma is to be investigated.Methods A case-control study was carried out in 18 asthmatics and 15 healthy subjects.The insertion/deletion (I/D) polymorphism of ACE gene was amplified by polymerase chain reaction (PCR).Results There was a higher gene frequency of DD genotype of ACE gene and D alleles in asthmatic subjects compared with healthy subjects (61% vs 20%,and 72% vs 37%,P<0.05).Conclusion The results suggested that DD allele of ACE genotype was significantly involved in genetic susceptibility to asthma and might be a risk factor for asthma.