高效液相色谱法测定肝力保口服液中芍药苷含量

Determination of paeoniflorin in Ganlibao oral liquid by HPLC

目的:测定肝力保口服液中芍药苷含量.方法:样品以甲醇直接提取,采用反相高效液相色谱法(HPLC)测定;流动相:甲醇-水(23:77);色谱柱:μBondapak C18;检测波长:230 nm;流速:0.8 ml/min.结果:空白试验阴性无干扰,精密度试验RSD为0.89%;加样回收率(94.32±4.38)%,加样回收率精密度RSD为1.95;线性范围12.5～400μg/ml.结论:本方法特异性高、精密、准确,可用于肝力保口服液的质量控制.

Objective: The content of paeoniflorin in Ganlibao oral liquid was determined by HPLC. Methods: Paeoniflorin was extracted with methanol. The HPLC system consisted of ∶77) mixture, with detection at 230 nm, flow rate 0.8 ml/min. Results: We did not find any interference peak from the blank test. The precision (RSD) was 0.89%. The mean recover of (94.32±4.38)% for paeoniflorin was obtained. The linear range was 12.5 ~ 400 靏/ml. Conclusions: This method is specific, accurate and reproducible.