7个绵羊微卫星DNA标记在绵(山)羊群体中的多态性检测

Investigation on polymorphism of 7 sheep microsatellite markers in sheep (goat) populations

选择分别位于绵羊2,4,6,9,17和19号染色体上的7个微卫星标记OarFCB11,OarFCB128,MAF70,OarAE101,MAF33,OarFCB48和OarFCB304,对湖羊、同羊及长江三角洲白山羊的典型群随机样本相应位点进行了PCR检测。结果表明,每个微卫星座位发现7个以上等位基因,且均存在多态;湖羊、同羊、长江三角洲白山羊群体杂合度(H)分别为0.9092,0.9177和0.8867,相应的群体基因平均多态信息含量(PIC)分别为0.9024,0.9116和0.8774,有效等位基因数(Ne)分别为11.7723,12.4538和11.6104,均以同羊为最高;以产生有效条带为标志,随机样本的PCR扩增效率存在明显的群体间和座位间差异,以长江三角洲白山羊和OarFCB128座位为最高;以7个座位微卫星等位基因频率推算群体间标准遗传距离,山羊与绵羊群体间遗传距离远大于绵羊群体之间,湖羊、同羊间亲缘距离系数R为0.1998。研究结果提示:选择的7个微卫星DNA座位均为多态座位;以7个微卫星标记为测度,湖羊、同羊及长江三角洲白山羊具有丰富的遗传多态性;7个微卫星标记可进一步用于绵山羊近缘种间遗传分析研究。

7 sheep microsatellite markers (OarFCB11,OarFCB128,MAF70,OarAE101,MAF33,OarFCB48 and OarFCB304) located on chromosome 2,4,6,9,17 and 19 respectively,were selected for examining the samples of Hu sheep,Tong sheep and Yantse River Delta White Goat (Csb) through PCR amplifying.The results displayed that:There are more than 7 alleles for each microsatellite locus.Mean heterozygosity (H) for Hu sheep,Tong sheep and Csb goat reached 0.909 2,0.917 7 and 0.886 7 respectively.The mean polymorphism information contents (PIC) for Hu sheep,Tong sheep and Csb goat was 0.902 4,(0.911 6) and 0.877 4 separately.Mean effective number of alleles (Ne) was 11.772 3,12.453 8 and (11.610 4).Tong sheep had highest level on above 3 indexes.By the standard of producing effective band,there were obvious difference in efficiency of PCR amplifying among populations and loci.Csb population and OarFCB128 locus had highest efficiency of PCR amplifying.The standard genetic distances between populations were computed based on alleles frequency of 7 microsatellite loci.The genetic distances between sheep and goat were much larger than between sheep populations.The phylogenetic distance coefficient (R) between Hu sheep and Tong sheep was 0.199 8.This study indicates:All 7 sheep microsatellite loci displayed high polymorphism;There were rich genetic variations existed in Hu,Tong sheep and Csb goat according to the analysis of the 7 microsatellite loci;The 7 microsatellite loci can be used for research on genetic differentiation between sheep and goat.