^(13)C-NMR分析漆酶处理木素的结构特征

Analysis of Laccase Treated Lignin Structure by 13C-NMR

利用1 3C NMR技术对漆酶、漆酶 /介体体系处理前后枫香木酶解木素进行结构分析。结果表明 ,经漆酶 /介体体系 (LMS)处理后 ,木素的部分 β O 4结构发生醚键断裂 ,脱甲基或脱甲氧基反应发生 ,根据结构变化推断反应过程中紫丁香基型结构优先反应 ;经漆酶处理后 ,木素单位苯环的羰基含量和甲氧基含量增加。

CEL of sweet gum before and after the treatment with laccase and LMS is analyzed by 13C-NMR method. It is found that ether bonds in β-O-4 type structure break down partly, and demethylation or demethoxylation occurs, and syringyl structure is preferential to take part in the reaction after LMS treatment. On the other hand, C=O content and -OCH 3 content in lignin increase after laccase treatment.