油溶性茶多酚—茶多酚脂肪酸酯的研制

Studies on the Preparation of Soluble Tea Polyphenol-Palmitate-Oil

将含量约87%的普通茶多酚同丙酸酐、棕榈酸酰氯、油酸酰氯溶于乙酸乙酯中,在碱性催化剂催化下进行非水相酯化反应,得到的产品经油溶性、抗氧化性等指标比较知以茶多酚棕榈酸酯效果较佳。经过单因素及正交试验,确定了最佳合成条件为:茶多酚/棕榈酸酰氯/催化剂N为1:3.0:2.5(摩尔比),茶多酚/乙酸乙酯为1:40(W/V),反应温度40℃,时间8h。在此条件下得到的茶多酚棕榈酸酯产品的产率为85.24%,Rancimat的诱导时间290min,抗氧化保护系数为1.89,产品为淡黄色,200×10-6添加量时不改变油脂的澄清透明度,浊度与折光指数均与空白对照基本一致。用ESI质谱分析,初步判断产品中含有几种儿茶素的一酯、二酯和少量未反应的儿茶素及棕榈酸。

The reaction products between the common Tea Polyphenols (TP) and propiomic anhydride or palmitoyl chloride or oleinoyl chloride in ethyl acetate solution (with alkaline catalyst) were evaluated by the antioxidative activity and solubility in refined edible oil, while the Tea Polyphenol Palmitate (TPP) showed the best result. The optimized synthesis conditions through further studies were as following : TP/ palmitoyl chloride/alkaline catalyst N=1:3.0:2.5 (mole), TP/ethyl acetate=1:40(W/V), 40℃, 8h. Under there conditions the light yellowish product was obtained. Its yield was 85.24%, the Rancimat (120℃) Induction period (IP) was 290min whereas control refined rapeseed oil was 154min. The Protection Index (PI) was 1.89. The transparency, turbidity value and diopter were showing no difference between the oil contained 200× 10-6 TPP and the oil control. It was preliminary concluded by Electric Spray Ionization Mass Spectrum (ESI-MS) that TPP contained the mono-ester, di-ester of several catechins, and some non-reactioned catechins and palmitic acid.