摘要
建立了RP-HPLC法分离检测大豆黄酮和染料木素的方法。采用的色谱条件为:WatersSpherisorb?ODS2柱(4.6mmi.d.×150mm,5μm),以甲醇-水(体积比为60:40)为流动相,流速为0.7ml/min,检测波长为254nm。大豆黄酮和染料木素日间拟合工作曲线的线性范围分别为0.05～20μg/ml和0.05～10μg/ml,相关系数分别为0.9984(p<0.01)和0.9978(p<0.01)。大豆黄酮和染料木素的加标回收率分别为91.72%～98.56%和96.80%～98.60%,日内、日间精密度分别为1.30%、5.30%和1.17%、3.84%。样品实测值的重复性范围分别为2.15%～5.99%和2.47%～7.17%。探讨了不同生物样品(蛋黄、肌肉、饲料、血清和肝脏)的预处理方法,比较了超声波法、有机溶剂热回流法和冷浸法,结果发现以超声波法提取样品中大豆黄酮和染料木素的提取率最高,操作亦较简易。
A reverse-phase high performance liquid chromatographic method was established for the determination of daidzein and genistein. Waters Spherisorb? ODS2 column(4.6mm i.d.× 150mm, 5μ m) was used with a mixture of methanol- water (60:40, V/V) as the mobile phase and a detector set at 254nm. The calibration curve was linear(r=0.9984, p< 0.01) in the range of 0.05～20μg/ml of dadizein and was also linear(r=0.9978, p< 0.01) in the range of 0.05～10μg/ml of genistein. The recoveries of dadidzein and genistein were 91.72%～98.56% and 96.80%～98.60% respectively with the intra-day and inter-day RSDs of 1.30%、5.30% and 1.17%、3.84%, respectively. The pretreatment methods of different bio-samples were compared. It was found that ultrasonic treatment was the best among the three methods.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第12期143-146,共4页
Food Science
基金
国家自然基金资助项目(39970534)
教育部优秀青年教师基金项目(E200001)
