电磁辐射对大鼠睾丸合成睾酮功能的影响

EFFECT OF ELECTROMAGNETIC IRRADIATION ON FUNCTION OF TESTOSTERONE SYNTHESIS IN RAT TESTIS

目的探讨电磁辐射对成年大鼠睾丸组织中类固醇合成急性调节蛋白 (StAR)、细胞色素P4 5 0胆固醇侧链裂解酶 (P4 5 0scc)的作用。方法采用放射免疫方法分别测定电磁辐照后 3、6、2 4、72h及对照组血清睾酮含量 ,同时运用RT -PCR方法测定睾丸组织中StARmRNA、P4 5 0sccmRNA水平。结果辐照后 3h血清睾酮含量和StARmRNA、P4 5 0sccmRNA水平分别较对照组降低 83.1%和 5 7.3%、5 3.6 % ,(P <0 .0 1) ;6h略有回升 ,但仍分别较对照组降低 5 2 .6 %、17.9%、2 9.2 % (P <0 .0 1,P <0 .0 5 ) ;2 4h恢复到正常水平 ,72h再次分别较对照组降低 5 7.6 %、39.5 %、5 3.5 % (P <0 .0 1)。结论电磁辐射能在转录水平影响睾丸间质细胞StARmNA、P4 5 0sccmRNA的表达 。

Objective To study the effect of electromagnetic irradiation on steroidogenic acute regulatory protein (StAR) and cytochrome P450 cholesterol side chain clevage enzyme (P450 scc) in adult rat testis tissue.Methods The contents of testosterone in serum of rats at 3,6,24,72 h after electromagnetic irradiation and control rats were measured by using radioimmunoassay(RIA), meanwhile the levels of StAR mRNA and P450scc mRNA in testis tissue were measured by using semi-quantitative RT-PCR. Results The levels of testosterone, StAR mRNA and P450scc mRNA at 3 h after electromagnetic irradiation decreased 83.1%,57.3%,53.6% more respectively than those in control rats,(P<0.01). At 6 h, although those had a little elevation, still decreased 52.6%,17.9%,29.2% more respectively than in control rats ,(P<0.01,0.05), and recovered at 24 h. At 72 h, the levels of testosterone, StAR mRNA and P450scc mRNA fell again (decreased by 57.6%,39.5%,53.5% respectivly,P<0.01). Conclusion Electromagnetic irradiation may affect the transcription of StAR and P450scc in rat Leydig cells,thereby decrease testosterone synthesis.