复合型生物衍生组织工程骨的制备及其理化特性

Experimental studies on procedures and properties of composite derived material of heterogenous bone

目的我们用物理化学方法处理自制的天然生物衍生骨支架材料—犬脱抗原松质骨(AECB),并复合具有成骨活性的骨形态发生蛋白(BMP)和具有成血管作用的碱性成纤维细胞生长因子(bFGF),制成复合型生物衍生组织工程骨。测其理化性质及力学强度,为组织工程研究提供可供选择的植骨材料。方法将犬松质骨进行一系列理化处理,制成脱抗原松质骨,用化学方法分析其组成成分,用扫描电镜观察其形貌特征,用X线能谱仪测定其钙磷比值,并对材料的力学性能进行分析测定,然后用物理方法在材料上复合BMP和bFGF,再用扫描电镜观察其复合情况。结果犬脱抗原松质骨脱钙及有机质洗脱行之有效,保持原骨天然网状孔隙系统及一定力学强度,并且BMP和bFGF能与其有效结合。结论犬脱抗原松质骨BMPbFGF有望为骨缺损、骨坏死的治疗开辟一条新途径,尚需要检测其免疫原性及成骨、成血管能力。

Objective To detect the properties of composite derived material of canine antigen-extracted cancellous bone which was treated with different physical and chemical method, and was mixed with osteoinductive BMP and angiogenerable bFGF. Methods We made antigen-extracted cancellous bone from canine.Their morphological features, constitute components, ratios of calcium to phosphorus and mechanical properties were examined by scanning electron microscopy, X-rays diffraction analysis, mechanical assay and so on. At the same time, the morphological features of composite derived material were examined by scanning electron microscopy also. Results The calcium-extracted and organic-extracted process was available. The antigen-extracted cancellous bone possess natural network pore system. The BMP and bFGF were seen in the network pore system.Conclusion Canine antigen-extracted cancellous bone/BMP/bFGF would be a useful composite material in treating bone defect and osteonecrosis, but more inverstigations will be necessary to detect its immunogenicity,bone induction and angiogenesis.