动物源食品中残留克伦特罗检测抗体的制备与应用

Preparation and application of clenbuterol antibody

采用重氮化法将盐酸克伦特罗 (CL)与牛血清白蛋白 (BSA)交联 ,用紫外扫描和凝胶电泳对交联物鉴定后免疫新西兰纯毛白兔 ,制备抗血清。经鉴定 ,抗血清中含有针对CL的抗体 ,效价为 3.2× 10 5;以此抗体为基础 ,设计了间接酶联免疫法 ,该法的检出限为 0 .0 96ng/mL ,对加于尿液、血液和组织中CL的回收率为 80 %～ 12 0 % ;通过重复性、灵敏度、准确性等指标对该法进行了评估 ,并与高效液相层析 (HPLC)及国外进口试剂盒进行了比较 ,表明该法的各项参数均能满足实际检测的需要。

Clenbuterol (CL)was coupled with bovine serum albumin by diazotization to synthesize immune antigen. After identification by ultra-violetscan andSDS denatured protein gel electrophoresis, the CL-BSA conjugate was used to immunize New Zealand rabbit. The antibody against clenbuterol was obtained from the rabbit antiserum. Its titre was 1∶3.2×10^(5). The clenbuterol antibody was used for ELISA kit preparation and it was showed that the minimum detection value was 0.096 ng/mL. Some parameters such as reproducibility, sensitivity ,precision, accuracy were evaluated. The results indicate that the kit can be applied to actual detection.