小鼠小花棘豆生物碱中毒的病理学观察

Pathological observation of Oxytropis glabra alkaloidosis in mice

将 32只小鼠随机分成 4组 ,包括 3个试验组和 1个对照组。试验组每日分别按剂量4 0 0、80 0、16 0 0mg/kg给小鼠灌服小花棘豆生物碱正丁醇萃取部分 (粗碱 )水溶液 ,同时给对照组灌服等量的生理盐水。 6 0d后 ,采集心、肝、肾、脾和脑进行病理组织学及超微结构检查。结果发现 ,肝、肾等实质器官的细胞胞质空泡变性 ,这与苦马豆素中毒引起的病理变化一致 ,表明小花棘豆的主要有毒成分是苦马豆素。

Effects of Oxytropis glabra alkaloids were investigated in mice. 32 mice were divided into 4 groups, including one control group and three experimental groups that received 1600,800 and 400 mg of crude alkaloids per kgof live weight per day for 60 days respectively. Fragments from some tissues of the mice were collected for histopathological observation. It was found that the histopathological lesions were characterized by the vacuolar degenerations in the liver, pancreas, thyroid and kidney cells, and in the neura of the central nervous system of the mice received the crude alkaloids. All these alterations occurred in a dose-dependent manner.The histopathological changes were similar to that of the toxicity caused by swainsonine and it was concluded that the main alkaloid of Oxytropis glabra may be swainsonine.