摘要
从高效杀虫植物闹羊花 (RhododendronmolleGDon)中分离获得 13个木藜芦烷类化合物 ,活性测定结果表明 ,化合物 1(RhodojaponinIII)和 6 (RhodomolleinI)对萝卜蚜无翅成蚜具有较高的忌避活性 ,以 2 0mg/L处理后 2 4h ,忌避率分别为 76 .6 4 %和 6 5 .0 6 % ,化合物 1、4 (RhodomolleinXVI)、6以 5mg/L处理后 ,与CK相比 ,试虫实验种群增殖倍数分别仅为 0 .2 1、0 .31和 0 .2 7;化合物 1、4、8(RhodomolinC)和 10 (RhodomoleinXVIII)浸叶饲喂法处理后 4 8h ,对萝卜蚜无翅成蚜的LC50 分别为 11.6 7、2 0 .14、2 4 .36、2 6 .92mg/L ,毒杀活性明显高于其他供试化合物。构效关系分析表明 ,保留C2 -C3 -环氧基 ,C6保留OH取代或降低其电子云密度 ,C10 保留单键取代基 ,C14 引入适当酯基或其他基团增加其电子云密度 ,均有利于提高其活性。
Thirteen grayane diterpenoids (1~13) were previousl y i solated from Rhododendron molle G Don. Results showed that compound 1(rhodoj aponin III) and 6(rhodomollein I) possessed higher deterrent activity to the win gless-adult of turnip aphid, Lipalus erysiml (Kaltenbach), with 76.64% and 65.06% of repellen rate 24 h after treatment with 20 mg/L, and compound 1, 4 (rh odomollein XVI) and 6 could reduce relative multiplication time of tested popula tion and got 0.21, 0.31 and 0.27 times of CK reproduction when female adult aphi d treated with 5 mg/L, respectively. Compound 1,4, 8 (rhodomolin C) and 10 (rhod omolein XVIII) possessed significant higher toxicity than other tested compounds and got 11.67, 20.14, 24.36 and 26.92 mg/L of LC 50 values against wingles s-adult aphid 48 h after treatment, respectively. Analysis on preliminary struc ture-activity relationship showed that substitute group of C 2-C 3-e poxy, C 6-OH, C 10 and C 14,which would was most important to ins ecticidal activity of grayanoid diterpenoids. Both reduce the density of electro n cloud in C 6 and increase that in C 14 would improve their bioactivi ty. Ring A and B may be necessary to maintain high activity in this kind of comp ounds. These results would give much valuable information to optimize the chemic al structure of grayane diterpenoids.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2004年第6期620-625,共6页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目 (3 0 2 70 886)和广东省自然科学基金项目 (0 10 3 0 1)资助
关键词
木藜芦烷类化合物
闹羊花
萝卜蚜
生物活性
构效关系
grayane diterpenoids
Rhododendron molle
Lipalu s erysiml (Kaltenbach)
insecticidal activities
structure-activity relations hip