摘要
目的探讨金属基质蛋白酶-2(MMP-2)及转化生长因子β1(TGF-β1)在人脑胶质瘤中的表达及意义。方法采用免疫组化方法对MMP-2和TGF-β1在30例胶质瘤和8例正常脑组织中的表达进行检测。结果MMP-2在正常脑组织中几乎不表达,在大多胶质瘤中均有表达,其表达水平随着胶质瘤恶性程度的增高而增高,且在正常脑组织和低(Ⅰ-Ⅱ级)、高(Ⅲ-Ⅳ)级别胶质瘤组中的表达差异显著(P<0.05);TGF-β1亦如此。同时,MMP-2与TGF-β1的表达水平呈正相关(r=0.76,P<0.01)。结论MMP-2与TGF-β1蛋白的阳性表达与肿瘤细胞病理学分级密切相关,它们参与了在人脑胶质瘤细胞的恶性转化,两者之间的协同作用可能在胶质瘤细胞的增殖中具有重要意义。
Objective To investigate the expressions and effects of matrix metalloproteinase-2 (MMP-2) and transform growth factor-β1 (TGF-β1) in human brain gliomas. Methods The expressions levels of MMP-2 and TGF-β1 in 30 cases of human gliomas and 8 specimens of normal brain tissue were detected by immunohistochemical technique. Results The positive expressions levels of MMP-2 (27.81%±8.54%) and TGF-β1 (18.22%±7.84%) in the gliomas of grades Ⅰ-Ⅱwas significantly higher than those (MMP-2: 5.78%±3.21%, TGF-β1: 8.36%±5.26%) in the normal brain tissue (P<0.05), and significantly lower than those (MMP-2: 42.71%±13.25%, TGF-β1: 39.42%±14.83%) in the gliomas of grades Ⅲ-Ⅳ (P<0.05). The expression of MMP-2 was positively correlated with that of TGF-β1 (P<0.01). Conclusion MMP-2 and TGF-β1 play the important roles in the malignization of human brain glioma.
出处
《中国临床神经外科杂志》
2004年第6期414-416,共3页
Chinese Journal of Clinical Neurosurgery
