摘要
目的分析MT1-MMPmRNA和CD44蛋白的表达与脑胶质瘤恶性程度的关系。方法采用原位杂交和免疫组化方法检测42例人脑胶质瘤和8例正常脑组织中,CD44s蛋白和MT1-MMPmRNA的表达。结果①CD44s蛋白表达在Ⅲ-Ⅳ级脑胶质瘤与在正常脑组织、Ⅰ～Ⅱ级脑胶质瘤之间有显著的差异(P<0.01);②MT1-MMPmRNA的表达水平与肿瘤的级别呈正相关(P<0.01);在Ⅲ-Ⅳ级脑胶质瘤与在正常脑组织、Ⅰ～Ⅱ级脑胶质瘤之间其表达均有显著性差异(P<0.01)。③CD44s蛋白表达与MT1-MMPmRNA表达呈正相关(r=0.895,P<0.01),二者均与肿瘤的恶性程度呈正相关(P<0.01)。结论CD44s和MT1-MMP与脑胶质瘤的发生发展密切相关。
Objective To explore the correlation between the expressions of MT1-MMP mRNA and CD44s, and the degree of gliomas malignance. Methods The expressions of CD44s protein and MT1-MMP mRNA were determined by immunohistochemistry and in situ hybridization in 42 cases of human gliomas and 8 case of normal brain tissue. Result ①The CD44s expression was significantly higher in grade Ⅲ-Ⅳ gliomas than those in gradeⅠ～Ⅱ gliomas and normal brain tissue(P<0.01). ②The expression of MT1-MMP mRNA was significantly higher in grade Ⅲ-Ⅳ gliomas than those in grade Ⅰ～Ⅱ and normal brain tissue (P<0.01). ③CD44s protein expression was positively related with MT1-MMP mRNA expression (r=0.895, P<0.01) and both of them were positively related with the degree of tumor malignance. Conclusion CD44s and MT1-MMP were closely involved in gliomas pathologenisis and development.
出处
《中国临床神经外科杂志》
2004年第6期420-421,424,共3页
Chinese Journal of Clinical Neurosurgery
