IL-1ra对兔晶状体囊外摘除术后血液泪液中IL-1含量影响

Effect of IL-1ra on the IL-1 content of blood and tears in rabbit after ECCE operation

目的 兔眼行晶状体囊外摘除术并给予IL 1ra后 ,检测兔血液、泪液中IL 1含量的动态变化 ,探讨IL 1对术后眼内炎症反应的影响。方法 哈白兔 4 0只 ,随机分为 5组 :对照组 (单纯行晶状体囊外摘除术 )、结膜下给药组、前房内给药组分 3组 ,2 5 μg ml、5 0 μg ml、10 0 μg ml。术前、术后 3天、7天、14天、2 8天取兔耳缘静脉血及泪液 ,采用放射免疫 (RIA)法检测IL 1含量。结果 给药组兔血液、泪液中IL 1含量在术后 3天、7天低于对照组 ,前房内给药的 3组IL 1含量呈剂量依赖性 ,术后 2 8天血液、泪液中IL 1的含量基本恢复到术前水平。结论 晶状体囊外摘除术后血液、泪液中IL 1在术后早期的炎症反应中作为炎症介质 ,起着重要作用 ,可以应用IL 1ra拮抗IL 1在术后早期的炎症反应。

Objective To measure the trands change of IL-1 content of blood and tears,and inquire into the effect of IL-1 on ocular inflammation.Methods Forty rabbits were randomly divided into five groups.Normal control group(Rabbit eyes were only given ECCE surgery),injected subconjunctiva group,and injected into anterior chamber group (include 25μg/ml,50μg/ml,100μg/ml three groups).Collectd blood and tears at preoperation,after operation 3 days,7 days,14 days and 28 days,using RIA method examined IL-1 content.Results The conditions of giving IL-1ra groups's density in blood and tears was lower than control groups' s density after operation 3 days,7days,three groups injected into anterior chamber's density depended on dose.The IL-1 content of blood and tears were normal after operation 28 days.Conclusion After ECCE,IL-1 in blood and tears has an important effect on preinflammation reaction as inflammation matter after operation.Preinflammation reaction after operation could use IL-1ra to restrain IL-1.