中药黄芪对脊髓星形胶质细胞单核细胞趋化蛋白-1分泌的影响

Effects of astragalus root on the secretion of MCP-1 by astrocytes in vitro from spinal cord of adult rats

目的 :研究黄芪对体外培养的脊髓星形胶质细胞趋化蛋白 1(MCP 1)分泌的影响。方法 :自Wistar大鼠脊髓组织分离、纯化星形胶质细胞 ,体外培养 ,分别予以TNF α及TNF α+黄芪处理 ,ELISA方法检测培养上清液中MCP 1的表达。结果 :TNF α可以显著刺激星形胶质细胞合成、释放MCP 1,而黄芪可下调其刺激作用。结论 :受炎症因子刺激活化的星形胶质细胞可能是损伤脊髓局部MCP 1的来源之一 ,黄芪可以减少脊髓损伤后内源性MCP 1的产生 ,从而缓解继发性脊髓损伤 ,发挥脊髓保护作用。

Objective:To study the secretion of MCP-1 by astrocytes culturted in vitro and the possible influence of astragalus root(AR).Methods:Astrocytes were separated and purified from spinal cord of adult Wistar rats.The expression of MCP-1 was detected by ELISA when the astrocyte were culturted in vitro with TNF-α or combined with AR.Results:TNF-α markedly increase the secretion of astrocyte-derived MCP-1 which could be down-regulated by AR to some extent.Conclusion:Astrocytes activated by some inflammatory factors may be one of the sources of MCP-1 secreted in the local injuried spinal cord.AR probably can decrease the engenous expression of MCP-1 and thus show a neuroprotective effects.