维持性血液透析患者外周血单个核细胞NF-κB活性水平观察及其对白介素-6表达的影响

The level of NF-κB activity in peripheral blood mononuclear cells in maintained-hemodialysis patients and its influence on expression of interleukin-6

目的 观察维持性血液透析 (MHD)患者PBMCNF -κB的活性水平 ,探讨其对PBMCIL - 6表达的影响。方法 使用同种膜透析器超过 6个月的MHD患者 33例 ,其中二醋酸纤维素膜 (CDA膜组 ) 17例 ,聚砜膜 (PS膜组 ) 16例 ,于单次血液透析前采血 2 5mL ,培养PBMC ,RT -PCR法检测IL - 6mRNA ,电泳迁移率改变实验测定NF -κB活性。结果 单纯培养基培养和LPS刺激下 ,单次血液透析前CDA膜和PS膜组MHD患者PBMCNF -κB活性、IL - 6的蛋白质和mRNA表达均显著高于健康人和非透析CRF患者 (P <0 .0 5 ) ;NF -κB特异性阻抑剂吡咯啉烷二甲基硫脲对各组患者PBMCNF -κB活性、PBMCIL - 6蛋白质和mRNA表达均有明显的抑制作用 (P <0 .0 1) ;MHD患者PBMCNF -κB活性与IL - 6蛋白质和mRNA的表达呈正相关。结论 MHD患者PBMCNF -κB活性增高 ,且可能参与了PBMCIL - 6高表达的调控。

Objective To investigate the level of PBMC NF-κB activity,and its influence on expression of interleukin-6 in maintained-hemodialysis (MHD) patients. Methods Thirty-three patients were included in the study,17 cases received hemodialysis with cellulose diacetate membrane (CDA group) and 16 cases with polysulfone membrane (PS group),for more than 6 months. Fresh isolated PBMC were cultured. IL-6 mRNA were measured with RT-PCR,and activity of NF-κB were measured with eletrophoresis mobility shift assay. Results Cultured with medium or lipopolysaccharide,the level of PBMC NF-κB activity,gene expression and protein synthesis of PBMC IL-6 in PS and CDA group were significantly increased when compared with those in healthy and non-hemodialysis group (P<0.05). Pyrrolidinedithiocarbamate could not only significantly inhibit the activity of NF-κB,but also inhibit the expression of IL-6 in PBMC (P<0.01). The level of in NF-κB activity PBMC correlated with gene expression and protein synthesis of IL-6 in PBMC (P<0.05). Conclusion We suggest that the level of PBMC NF-κB activity is increased,and NF-κB may mediate IL-6 up-expression in MHD patients.