加压灌注修复家兔脑梗死后缺血组织的实验研究

The experimental study on enhancing perfusion for repairing ischemic tissue after cerebral infarction in rabbits

目的 检验加压灌注治疗非急性脑梗死的疗效 ,探讨加压灌注治疗的理论依据。方法 采用开颅法制备兔大脑中动脉闭塞模型 ,根据给药方法不同将家兔分为加压灌注治疗 (A)组、治疗对照 (B)组、梗死对照 (C)组和正常对照 (D)组。 72h后对A组经颈动脉内加压快速注入尿激酶溶液 ,对梗死后兔的行为学、受损脑组织形态、大小变化进行研究 ,并对梗死区的神经元细胞进行凋亡研究。结果 A组症状改善明显 ,有效率达 83.3% ,与之对应 ,A组梗死周边区的神经元凋亡数目与B、C组相比较差异有显著性意义 (P <0 .0 5 )。结论 加压灌注治疗能有效干预非急性血栓性脑血管病 ,为非急性血栓性脑血管病临床治疗提供一种全新的方法和思路。

Objective To investigate the therapeutic effect and the principle of enhancing perfusion for the treatment of non-acute cerebral infarction.Methods Focal cerebral infarction was produced by obstructing middle cerebral artery by means of operation in rabbits.The rabbits were divided into four groups according to different methods of treatment. 72 hours after infarction,group A was given catheterization of the internal carotid artery and quick injection of urokinase,then the behavior of the rabbits,the ischemic area,pathological changes and the histological feature of apoptotic neuron death of the ischemic cerebral tissues were studied.Results The clinical symptoms improved obviously in enhancing perfusion group(effective rate=83.3%).At the same time ,the number of apoptotic neurons in area surrounding the ischemic core in group A was lower than that in groups B and C(P<0.05). Conclusion The treatment with enhancing perfusion was effective for the non-acute cerebral infarction,which provides a new method and idea for the treatment of non-acute cerebral infarction.