紫花苜蓿品种根瘤菌表型多样性研究

Phenotype Diversities of Rhizobia Isolated from Different starins of Medicago sativa

选用 5 2株分离自 2 2个紫花苜蓿品种的根瘤菌和 34株已知参比菌株 ,进行生长温度、营养利用、抗生素抗性和耐逆性等 113个表型性状研究 ,结果表明 :NWMSH0 2 2、NWMSH0 2 3和 NWMSH0 2 8这 3株菌抗逆性较强 ;通过MINTS软件分析 ,得到数值分类树状图 ;在 89%的相似水平上 ,除 NWMSH0 0 9和 NWMSH0 2 3,供试未知菌株聚为不含已知参比菌株的新表观群 。

A study of 113 phenotype features, including plant-growing temperature, nutrition utilization, antibiotic resistance, and NaCl and alkali tolerance, of 52 strains of Medicago sativa, isolated from the rhizobia of 22 M.sativa strains and 34 representative strains, was conducted. The result shows that the three strains: NWMSH022, NWMSH023, and NWMSH028 boast stronger NaCl and alkali tolerance than the others. By the analysis of MINTS software, a dendrogram of numerical taxonomy shows that, except NWMSH009, and NWMSH023, all the examined strains cluster into one phenotype at a similarity level of 89%. It takes a further genetic study to determine whether this new phenotype differs from the known rhizobial species.