系统性红斑狼疮患者外周血单个核细胞趋化因子受体4、5mRNA的表达

Studies on expression of chemokine receptors CCR4 and CCR5 mRNA in patients with systemic lupus erythematosus

目的探讨趋化因子受体CCR4及CCR5在系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中的表达,与疾病的相关性。方法收集93例确诊的SLE患者和30名正常对照,通过外周血单个核细胞(PBMC),提取RNA。应用反转录-聚合酶链反应(RT-PCR)方法检测研究对象CCR4、CCR5mRNA表达水平。结果趋化因子受体CCR4mRNA在SLE患者PBMC中的表达水平,患者组(1.57±0.70)与正常对照组(0.19±0.18)比较,两者差异有显著性(t=3.012,P=0.003);活动期(2.03±1.04)非活动期(0.26±0.19)及对照组比较,两者差异有显著性;非活动期与对照组比较,差异无显著性(P>0.05)。趋化因子受体CCR5mRNA在PBMC中表达水平,患者组(0.56±0.44)与对照组(0.37±0.14)比较,两者差异有显著性(t=3.262,P=0.002);活动期(0.53±0.51)与非活动期(0.59±0.34)比较,差异无显著性(P>0.05);活动期与对照组比较(t=2.039,P=0.045)及非活动期与对照组比较(t=3.461,P=0.001),两者差异有显著性。SLE患者组CCR4及CCR5mRNA水平与疾病活动度评分关系:CCR4mRNA水平与SLEDAI(r=0.376,t=3.851,P=0.000)呈正相关。CCR5mRNA水平与疾病的活动性(SLEDAI)(r=0.062,t=-0.589,P=0.557)不相关。结论CCR4mRNA表达水平在患者组比对照组表达增高,活动期比对照组表达水平增高。

Objective To monitor the expression levels of chemokine receptors mRNA (CCR4 and CCR5) in peripheral blood mononuclear cell (PBMC) from patients with systemic lupus erythematosus (SLE) and the relationship between the levels and disease activity. Methods Nighty-three definitive SLE patients and 30 controls were collected. PBMC were separated from the peripheral blood specimen. Reverse transcriptase polymerase chain reaction (RT-PCR) technique was applied to semi-quantitatively analyze the expression of chemokine receptor′s mRNA (CCR4 and CCR5) in PBMC. Results Level of CCR4 mRNA expression was significantly increased in patients with SLE compared with healthy controls, and significantly higher in patients with active SLE than those of the stable stage. Level of CCR4 mRNA expression in PBMC showed significantly positive correlation with disease activity(SLEDAI). Level of CCR5 mRNA expression was significantly higher in patients than controls and there was no significant difference between the active patients and the stable patients. The level of CCR5 expression showed no correlation with disease activity. Conclusions These results suggest that CCR4 and CCR5 may be involved in the pathogenesis of SLE, and the expression level may be a good indicator for the disease activity of SLE.