7-硝基吲唑对兔异丙酚麻醉深度调节的研究

Effects of 7-nitro indazole on anesthetic depths in rabbits induced by propofol

目的:研究7-硝基吲唑(7-nitro indazolei,,7-NI)对兔异丙酚麻醉深度的调节作用。方法:20只日本大耳兔,雌雄不限,随机分为2组(n=10):对照组经腹腔注射2 ml花生油;7-硝基吲唑组经腹腔注射50 mg·kg-17-硝基吲唑。20 min后经颈外静脉连接Graseby 3 500注射泵进行靶控输注(TCI),以咀嚼反射消失作为浅麻醉标志,测定达浅麻醉状态所需异丙酚TCI血药浓度、双频指数(BIS)值及心率变异指数(HRVI)值。对照组达浅麻醉状态后再次经腹腔注射50 mg·kg-17-硝基吲唑,观察BIS值、HR-VI值变化。结果:50 mg·kg-17-硝基吲唑显著降低兔达浅麻醉状态时所需TCI浓度。对照组达浅麻醉状态后再次注射50 mg·kg-17-硝基吲唑麻醉深度表现加深趋势,但无显著的统计学意义(P>0.05)。结论:7-硝基吲唑通过选择性抑制中枢nNOS活性增强异丙酚麻醉效能,提示中枢NO／cGMP信号转导途径在异丙酚全麻作用机制中发挥作用。异丙酚可能通过抑制中枢nNOS活性影响NO／cGMP信号转导系统。

AIM: To investigate the regulative effects of 7-NI on anesthetic depths in rabbits induced by propo-fol. METHODS: Twenty Japanese large auris rabbits of both sexes were randomly divided into 2 groups ( n = 10 in each) : control group was given 2 milliliter peanut oil, ip and trial group was given 50 mg·kg-1 7-NI, ip. After twenty minutes of administration, propofol were target-controlled infused by Graseby 3 500 injection pump that connected with jugular vein. The loss of chewing was indicated the light anesthesia. The blood concentration of propofol and the bispectral index ( BIS) , and heart rate variability index (HRVI) were determined as the light anesthetic depth reached. In order to observe the changes of BIS and HRVI, the control group was given 50 mg·kg-17-NI, ip in the light anesthetic depth. RESULTS: 50 mg·kg-1 7-NI significantly decreased the target blood concentrations in the light anesthetic depth. Administration of 50 mg·kg-1 7-NI increased the anesthetic depth after the light anesthetic depth reached (P > 0.05). CONCLUSION: 7-NI can increase the anesthetic action of propofol by selectively inhibition of the activity of central nNOS, indicated that central NO/cGMP signal trans-duction pathway play an important role in the general anesthesia machanism of propofol. Propofol may influence NO/cGMP signal transduction pathway by selectively inhibition of the activity of central nNOS.