摘要
目的 比较不同来源的造血干 /祖细胞表面归巢相关分子 (HRM)表达谱的差异性。方法 采用高度灵敏的四色流式细胞术检测脐血 (UCB)、动员后的外周血 (mPB)及骨髓 (BM)来源的造血干 /祖细胞表面系列HRM表达水平。结果 UCB、mPB及BM来源的CD34bright细胞均高度表达黏附分子CD4 4、CD11a、CD18、CD6 2L、CD31及CD4 9d ;但UCB来源的CD34bright细胞及CD34brightCD38-细胞黏附分子CD4 9e、CD4 9f、CD5 4及趋化因子受体CXCR 4的表达水平显著低于mPB及BM来源者 ;上述不同来源的造血干 /祖细胞均不表达其他趋化因子受体 ,包括CCR 1、CCR 2、CCR 3、CCR 5、CXCR 1、CX CR 2、CXCR 3及CXCR 5 ;更为有意义的是 ,只有mPB来源的CD34bright细胞表达基质金属蛋白酶MMP 2及MMP 9。CD34brightMMP 2 + 及CD34brightMMP 9+ 细胞百分率分别为 (11.4± 4 .9) %及 (2 7.6± 7.8) %。结论 UCB来源的造血干 /祖细胞低表达或不表达某些HRM ,这可能是UCB移植后造血重建延迟的原因之一。
Objective To compare the expression profiles of a set of homing related molecules (HRM) repertoire expressed on hematopoietic stem/progenitor cells (HS/PC) from different sources. Method The expression levels of HRM on HS/PC from umbilical cord blood (UCB), mobilized peripheral blood (mPB) and bone marrow (BM) were assessed using a highly sensitive 4 color flow cytometric analysis. Results UCB derived CD34 bright cells, as well as mPB and BM derived CD34 bright cells strongly expressed CD44 , CD11a, CD18, CD62L, CD31 and CD49d. On the other hand, significantly lower expressions of CD49e, CD49f, CXCR 4 and CD54 on UCB derived CD34 bright and CD34 bright CD38 - cells, compared with those on mPB and BM derived CD34 bright and CD34 bright CD38 - cells, were observed. None of UCB , mPB and BM derived CD34 bright cells expressed other chemokine receptors, including CCR 1, CCR 2, CCR 3, CCR 5, CXCR 1, CXCR 2, CXCR 3 and CXCR 5. Another striking finding was that only mPB derived CD34 bright cells expressed significant levels of both the matrix metalloproteinases MMP 2 [(11.4±4.9)%] and MMP 9 [(27.6±7.8)%]. Conclusion HS/PC from UCB have some defects of expression of HRM repertoire, which might partly explain the cause(s) of delayed hematopoietic reconstitution after UCB transplant.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2004年第12期736-739,共4页
Chinese Journal of Hematology
