分子标记在辅助甜瓜“A”基因转育上的研究

Primary Report on Research of Marker in Helping Gene A Transport of Melon

对甜瓜雄花两性花同株与雌雄异花同株材料间杂交后代及回交后代的花性型分离研究表明,F2群体中单性花性状呈现单显性基因控制的3∶1分离比例,证明供试材料雄花两性花同株与雌雄异花同株的基因型分别为aaGG和AAGG。并运用RAPD技术,在F2群体中采用混合分组分析法对A基因进行了分子标记筛选,找到一个与A基因连锁的大小约为500bp的RAPD标记。

Segregation patterns of flower types were analyzed in F_2 and BC_1 populations from each of two crosses between monoecious and andromonoecious materials of melon (Cucumis melo). Results showed that flower type difference between the two types of parents was controlled by a pair of genes at one single locus a, with AAGG and aaGG genotypes, respectively, as flower type segregation in F_2s and BC_1s from the two crosses was in agreement with simple Mendelian ratios of 3∶1 and 1∶1, respectively. RAPD primers were screened for polymorphisms between monoecious and andromonoecious gene pools from F_2 of Mr×YF cross using bulked segregant analysis (BSA) strategy. One polymorphic fragment (about 500 bp) was amplified, which will hopefully serve as a marker in marker-assisted selection in a backcross breeding programme for monoecious flower type.